Laser-induced Forward Transfer Hydrogel Printing: A Defined Route for Highly Controlled Process

Single cell isolation process with laser induced forward transfer

BACKGROUND A viable single cell is crucial for studies of single cell biology. In this paper, laser-induced forward transfer (LIFT) was used to isolate individual cell with a closed chamber designed to avoid contamination and maintain humidity. Hela cells were used to study the impact of laser pulse energy, laser spot size, sacrificed layer thickness and working distance. The size distribution,...

Laser Induced Forward Transfer for Materials Patterning

Today, a strong research effort is directed towards the development and integration of new materials, especially of polymers and biomolecules which are essential in pharmaceutical, bioengineering, and sensing applications. In fact, patterning of organic and inorganic materials, in particular enabling precise positioning of different materials with nano and micro scale resolution over large area...

Laser-induced Forward Transfer of Ag Nanopaste

Over the past decade, there has been much development of non-lithographic methods(1-3) for printing metallic inks or other functional materials. Many of these processes such as inkjet(3) and laser-induced forward transfer (LIFT)(4) have become increasingly popular as interest in printable electronics and maskless patterning has grown. These additive manufacturing processes are inexpensive, envi...

Laser-Induced Forward Transfer of Pure Metals

Highly Elastic Biodegradable Single-Network Hydrogel for Cell Printing

Cell printing is becoming a common technique to fabricate cellularized printed scaffold for biomedical application. There are still significant challenges in soft tissue bioprinting using hydrogels, which requires live cells inside the hydrogels. Moreover, the resilient mechanical properties from hydrogels are also required to mechanically mimic the native soft tissues. Herein, we developed a v...